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Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Article DOI: 10.3791/55894-v • 10:10 min • November 2nd, 2018
November 2nd, 2018 •
1,2, 1,2,3,4,5,6,7
1Research Service, VA Western New York Healthcare System, 2Department of Ophthalmology, (Ross Eye Institute), Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 3Pharmacology/Toxicology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 4Physiology/Biophysics, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 5Neuroscience Program, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo- SUNY, 6The RNA Institute, University at Buffalo- SUNY, 7The SUNY Eye Institute

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Summary
Automatic Translation

Here we demonstrate a novel approach to using high resolution spectral-domain optical coherence tomography (HR-SD-OCT) to assist delivery of gene therapy agents into the subretinal space, assess its areal coverage, and characterize photoreceptor vitality.

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Ultrahigh Resolution Mouse Optical Coherence Tomography Intraocular Injection Retinal Gene Therapy Research Vector Delivery Real-time Observation Gene Therapy Research Minimal Damage Injection Site Mapping Quantitative In Vivo Measurements Statistical Inference Anesthetized Mouse Retinal Imaging System Microscope Sterile Blunt Iris Forceps Eyelid Proptosis Corneal Limbus Edge Sterile PBS Solution Sterile Cover Slip Conjunctiva Scleral Depression Micro Manipulator
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