Journal
/
Medicine
/
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

DOI:
10.3791/55894-v

•

10:10 min

•

November 02, 2018

•

,
1Research Service,VA Western New York Healthcare System, 2Department of Ophthalmology, (Ross Eye Institute), Jacobs School of Medicine and Biomedical Sciences,University at Buffalo- SUNY, 3Pharmacology/Toxicology, Jacobs School of Medicine and Biomedical Sciences,University at Buffalo- SUNY, 4Physiology/Biophysics, Jacobs School of Medicine and Biomedical Sciences,University at Buffalo- SUNY, 5Neuroscience Program, Jacobs School of Medicine and Biomedical Sciences,University at Buffalo- SUNY, 6The RNA Institute,University at Buffalo- SUNY, 7The SUNY Eye Institute

Chapters

  • 00:04Title
  • 00:45Subretinal Injection
  • 02:37Injection Site and Subretinal Bleb Mapping
  • 04:03High Resolution Spectral-Domain Optical Coherence Tomography (HR-SD-OCT) Outer Retinal Degeneration Evaluation
  • 07:47Results: Representative Outer Retinal Change Quantification and Post-Subretinal Injection 3D Mapping
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here we demonstrate a novel approach to using high resolution spectral-domain optical coherence tomography (HR-SD-OCT) to assist delivery of gene therapy agents into the subretinal space, assess its areal coverage, and characterize photoreceptor vitality.

Tags

Ultrahigh ResolutionMouseOptical Coherence TomographyIntraocular InjectionRetinal Gene TherapySubretinal InjectionVector DeliveryTherapeutic Agent ToxicityMinimally InvasiveReproducibleReal-time ObservationRetinal ImagingProptosisScleral DepressionRetinal Pigment EpitheliumSubretinal SpaceInjection OutcomesOCT Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

Doppler Optical Coherence Tomography of Retinal Circulation

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Development of an Alpha-synuclein Based Rat Model for Parkinson’s Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Read Article