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JoVE Journal Developmental Biology
En standardiseret tilgang til multispecies Rensning af mammale mannlige germeller ved hjælp af mekanisk vævsdissociation og flowcytometri
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En standardiseret tilgang til multispecies Rensning af mammale mannlige germeller ved hjælp af mekanisk vævsdissociation og flowcytometri

Article DOI: 10.3791/55913-v • 08:46 min • July 12th, 2017
July 12th, 2017 •
*1,2,3,4, *1, 1, 1, 3,4, 1
1Department of Genetics, Washington University School of Medicine, 2Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, 3Instituto de Investigação e Inovação em Saúde, University of Porto, 4IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 5Department of Pathology & Immunology, Washington University School of Medicine
* These authors contributed equally

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Dette værk beskriver standardiseringen af ​​en metode til opnåelse af rensede kimcellepopulationer fra testikelvæv af forskellige pattedyrsarter. Det er en simpel protokol, der kombinerer mekanisk testis dissociation, farvning med Hoechst-33342 og propidiumiodid og FACS sortering, med brede anvendelser i sammenlignende undersøgelser af reproduktionstoksikologisk biologi.

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Udviklingsbiologi udgave 125 sammenlignende reproduktion fluorescensaktiveret cellesortering (FACS) Hoechst-33342 mandlige kønsceller mekanisk dissociation spermatogenese testis,
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