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Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

DOI:

10.3791/55928-v

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07:12 min

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September 28, 2017

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Galina Shapiro, Maxim Bez, Wafa Tawackoli^3,4, Zulma Gazit^2,3,5, Dan Gazit^2,3,4,5, Gadi Pelled^2,3,4

¹Skeletal Biotech Laboratory,Hebrew University-Hadassah Faculty of Dental Medicine, ²Department of Surgery,Cedars-Sinai Medical Center, ³Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, ⁴Biomedical Imaging Research Institute,Cedars-Sinai Medical Center, ⁵Department of Orthopedics,Cedars-Sinai Medical Center

Chapters

00:05Title
01:03Vertebral Defect Model
04:20MicroCT Scanning and Quantitative Structural Analysis
05:41Results: Longitudinal Analysis of Vertebral Defect Regeneration
06:35Conclusion

Summary

Automatic Translation

The goal of this protocol is to generate a nude rat osteoporosis-related vertebral compression fracture model that can be longitudinally evaluated in vivo using a semiautomated microcomputed tomography-based quantitative structural analysis.

Tags

Keywords: Semiautomated Longitudinal Microcomputed Tomography Quantitative Structural Analysis Nude Rat Osteoporosis Vertebral Fracture Model Glial Regenerative Medicine Bone Regeneration In Vivo Anesthesia Surgical Procedure Abdominal Cavity Lumbar Spine Vertebral Bodies

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