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Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
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JoVE Journal Developmental Biology
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

DOI:
10.3791/55988-v

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12:10 min

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June 19, 2017

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, ,
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, Institute of Medical Science, College of Medicine,The Catholic University of Korea

Chapters

  • 00:05Title
  • 01:08iPSC Generation from Cord Blood
  • 02:31EB Generation
  • 04:43Outgrowth Cell Induction from EBs
  • 05:45Chondrogenic Pellet Formation
  • 07:19Chondrogenic Pellet Characterization by Staining
  • 10:01Results: Relative Anaysis Results of Chondrogenic Pellets Generated from CBMC-iPSCs
  • 11:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we propose a protocol for chondrogenic differentiation from cord blood mononuclear cell-derived human induced pluripotent stem cells.

Tags

Chondrogenic PelletCord Blood-derivedInduced Pluripotent Stem CellsCartilage RepairIPSCsCell CultureCell CountingCentrifugationSendai VirusIPSC MediumE8 MediumEDTACell Harvesting

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