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Immunology and Infection
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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
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Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

DOI:
10.3791/55997-v

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08:43 min

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June 24, 2017

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, , , , ,
1Laboratory of Cellular & Molecular Biology, National Cancer Institute,National Institutes of Health, 2Laboratory of Single Molecule Biophysics, National Heart, Lung, and Blood, Institute,National Institutes of Health

Chapters

  • 00:05Title
  • 00:36Preparation of Fluorescent Nano-diamond-coated Coverslips
  • 02:49Activation of Jurkat T Cells
  • 04:15Imaging Activated Jurkat T Cells Using madSTORM
  • 07:00Results: Multiplexed Super-resolution Image of Proteins in an Activated T Cell
  • 07:54Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate a method to image multiple molecules within heterogeneous nano-structures at single molecule accuracy using sequential binding and elution of fluorescently labeled antibodies.

Tags

Keywords: T CellsMadSTORMSuper-resolution ImagingMultiplexed ImagingSingle Molecule Localization MicroscopyAlexa 647Poly-L-lysineFluorescent NanodiamondsAnti-CD3 AntibodyJurkat T CellsHBS Solution

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