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Meget multiplexeret, superopløselig billeddannelse af T-celler ved hjælp af madSTORM
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
DOI:

08:43 min

June 24, 2017

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Chapters

  • 00:05Title
  • 00:36Preparation of Fluorescent Nano-diamond-coated Coverslips
  • 02:49Activation of Jurkat T Cells
  • 04:15Imaging Activated Jurkat T Cells Using madSTORM
  • 07:00Results: Multiplexed Super-resolution Image of Proteins in an Activated T Cell
  • 07:54Conclusion

Summary

Automatic Translation

Vi demonstrerer en metode til at vise flere molekyler inden for heterogene nanostrukturer ved nøjagtighed af enkeltmolekyler ved hjælp af sekventiel binding og eluering af fluorescensmærkede antistoffer.

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