Journal
/
/
Omprogrammering primære fostervand og membran celler til Pluripotency i Xeno-fri betingelser
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
DOI:

09:34 min

November 27, 2017

, , , , , ,

Chapters

  • 00:05Title
  • 00:50Isolation of Primary Mesenchymal Stem Cells from Human Amnion
  • 02:31Harvesting Cultured Amniotic Fluid Stem Cells and Amniotic Membrane Stem Cells
  • 03:47Transfection with Reprogramming Plasmids
  • 05:35Picking Fully Reprogrammed Colonies for Clonial Expansion
  • 06:43Clonal Expansion and Maturation of iPSCs
  • 07:47Results: Morphological Progression of Cells During Reprogramming
  • 08:43Conclusion

Summary

Automatic Translation

Denne protokol beskriver omprogrammering af primære fostervand væske og membran mesenkymale stamceller til induceret pluripotente stamceller ved hjælp af en ikke-integrere episomal tilgang i fuldt kemisk definerede betingelser. Procedurer for udvinding, kultur, omprogrammering og karakterisering af de resulterende inducerede pluripotente stamceller af strenge metoder er beskrevet.

Related Videos

Read Article