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High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment
JoVE Journal
Genetics
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JoVE Journal Genetics
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment

DOI:
10.3791/56012-v

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07:26 min

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July 18, 2017

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, , , ,
1Department of Immunology,Reims University Hospitals, Robert Debré Hospital, 2Faculty of Medicine, LRN EA 4682,University of Reims Champagne-Ardenne, 3Department of Internal Medicine and Geriatrics,Reims University Hospitals, Maison Blanche Hospital, 4Faculty of Medicine, EA 3797,University of Reims Champagne-Ardenne

Chapters

  • 00:05Title
  • 00:58Blood and Body Fluid DNA Extraction
  • 03:05High-resolution Melting (HRM)-PCR Protocol
  • 04:57Results: Determination of the CR1 Length Polymorphism Genotype Using High-resolution Melting PCR
  • 06:01Conclusion

Summary

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Here, we describe an innovative method to determine complement receptor 1 (CR1) length polymorphisms for use in several applications, particularly the assessment of susceptibility to diseases such as Alzheimer's disease (AD). This method could be useful to better understand the role of CR1 isoforms in the pathogenesis of AD.

Tags

Keywords: Complement Receptor 1 Length PolymorphismHigh-resolution Melting PCRAlzheimer’s DiseaseGenotypingNeuroscienceDNA ExtractionProteinase KLysis BufferEthanolMembrane Column

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