Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Archana Vijaya Kumar; Carmen Donate; Beat A. Imhof; Thomas Matthes

doi:10.3791/56023

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Full Text

11,724 Views

10:52 min

March 30, 2018

DOI: 10.3791/56023-v

Archana Vijaya Kumar^1,2, Carmen Donate², Beat A. Imhof¹, Thomas Matthes²

¹Department of Pathology and Immunology,University of Geneva, ²Hematology Service,University Hospital, Geneva

Overview

This article discusses the establishment of a xenograft mouse model for mantle cell lymphoma (MCL), a challenging B cell disorder. The model aims to facilitate the study of MCL biology and the development of therapeutic strategies.

Key Study Components

Area of Science

Hematooncology
Xenograft models
Therapeutic development

Background

Mantle cell lymphoma is difficult to treat.
Establishing xenograft models for MCL is challenging.
Understanding MCL biology is crucial for developing therapies.
Patient-derived xenografts can provide insights into treatment responses.

Purpose of Study

To establish a xenograft model for MCL.
To develop treatment strategies for MCL.
To study the therapeutic outcomes of various treatment approaches.

Methods Used

Establishment of xenografts in mice.
In vivo studies of drug effects on human lymphoma cells.
Utilization of patient-derived cells for therapy assessment.
Application of the model to various lymphoma types.

Main Results

Successful establishment of MCL xenografts in mice.
Insights into the therapeutic effects of treatments on MCL.
Feasibility of studying patient-derived cells in vivo.
Potential application of the model to other lymphoma types.

Conclusions

The xenograft model is a valuable tool for MCL research.
It aids in understanding therapeutic responses in MCL.
This method can be adapted for studying various lymphomas.

Frequently Asked Questions

What is mantle cell lymphoma?

Mantle cell lymphoma is a type of B cell lymphoma that is challenging to treat.

Why are xenograft models important?

Xenograft models allow researchers to study human cancer cells in a living organism, providing insights into treatment effects.

What are patient-derived xenografts?

Patient-derived xenografts are models created by implanting human tumor cells into mice, allowing for personalized treatment studies.

How can this model help in therapy development?

The model helps researchers evaluate the effectiveness of different treatment strategies on human lymphoma cells.

Can this method be applied to other types of lymphomas?

Yes, the xenograft model can be adapted to study various lymphoma types beyond mantle cell lymphoma.

What challenges exist in obtaining patient-derived xenografts?

Obtaining patient-derived xenografts can be difficult due to the need for viable tumor samples and the complexity of the procedure.

Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.

The overall goal of this method is to establish a xenograft mouse model for mantle cell lymphoma, to develop treatment strategies for this disease. This method can help answer key questions in the field of hematooncology such as therapeutic outcome of different treatment strategies. The main advantage of this technique is that it allows us to study the effect of drugs on human lymphoma cells in vivo.

The implications of this technique extend towards therapy for mantle cell lymphoma because using this model, it is not only feasible but also convenient to study the effect of therapy on patient derived cells. Though this method can provide insight into therapeutic aspects of mantle cell lymphoma, it can also be applied to study different types of lymphomas. Generally, individuals new to this method will struggle because it is difficult to obtain patient derived xenografts.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

Xenograft Mouse Model Mantle Cell Lymphoma Hematooncology Therapeutic Outcome Patient-derived Xenografts B Cell Migration Density Gradient Media Mononuclear Blood Cells Phosphate Buffered Saline Cell Suspension Cell Pellet