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Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Chapters
- 00:05Title
- 01:23Preparation of Primary Peripheral Blood Mononuclear Cells by Density Gradient Separation
- 03:15Enrichment of B Cells Negative Selection
- 05:34Development of the Xenograft Mouse Model
- 06:07Chimerism Analysis of Tumor Cell Engrafted in Different Organs
- 07:43Results: Use of Flow Cytometry to Analyze the B Cell Enrichment in Mice Xenografts Obtained from Mantle Cell Lymphoma Patients
- 09:08Conclusion
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
