Journal
/
Medicine
/
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

DOI:
10.3791/56025-v

•

05:45 min

•

July 31, 2017

•

,
1Biomedical Engineering,University of Virginia

Chapters

  • 00:05Title
  • 00:58FFPE Sample Deparaffinization and Rehydration and Antigen Retrieval
  • 01:54Chromogenic Immunohistochemistry
  • 03:50Results: Staining Effective for Oligodendrocyte Quantification
  • 05:17Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol was developed to quantitatively identify tumor microenvironment components in glioblastoma patient resections using chromogenic immunohistochemistry and ImageJ.

Tags

Keyword Extraction: Quantitative ImmunohistochemistryCellular MicroenvironmentGlioblastoma ResectionsTumor MicroenvironmentChromogenic ImmunohistochemistryImage AnalysisAntigen RetrievalPermeabilizationBlockingPrimary AntibodiesHorseradish PeroxidaseDiaminobenzidine

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56025?article_type=v

Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
check_url/56025?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/56025?article_type=v

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy
check_url/56025?article_type=v

Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma
check_url/56025?article_type=v

A “Patient-Like” Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
check_url/56025?article_type=v

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
check_url/56025?article_type=v

Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling and Drug Response Testing
check_url/56025?article_type=v

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
check_url/56025?article_type=v

Quantitative [18F]-Naf-PET-MRI Analysis for the Evaluation of Dynamic Bone Turnover in a Patient with Facetogenic Low Back Pain
check_url/56025?article_type=v

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice

Read Article