Journal
/
Medicine
/
Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo

Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo

DOI:
10.3791/56028-v

•

10:39 min

•

March 08, 2018

•

, , , , , , , ,
1Institute of Microcirculation,Chinese Academy of Medical Sciences & Peking Union Medical College

Chapters

  • 00:04Title
  • 01:49Using Streptozotocin and Sugar-water to Make Mice Diabetic
  • 03:12Preparing and Calibrating the Laser Doppler
  • 04:24Laser Doppler Analysis of Microvascular Vasomotion in Pancreatic Islet
  • 07:56Results: Microvascular Vasomotion in the Pancreatic Islet of STZ-induced Diabetic Mice
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

Pancreatic islet microvascular vasomotion regulates islet blood distribution and maintains the physiological function of islet β cells. This protocol describes using a laser Doppler monitor to determine the functional status of pancreatic islet microvascular vasomotion in vivo and to assess the contributions of pancreatic islet microcirculation to pancreatic-related diseases.

Tags

Laser DopplerPancreatic IsletMicrovascular VasomotionIn VivoCirculatoryDiabetesSTZ InjectionBlood GlucoseOptical Probe

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Endothelin-1 Induced Middle Cerebral Artery Occlusion Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Rat

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment

Fundus Photography as a Convenient Tool to Study Microvascular Responses to Cardiovascular Disease Risk Factors in Epidemiological Studies

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model

Read Article