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JoVE Journal
Biochemistry

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Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
 

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Article DOI: 10.3791/56031 06:36 min
July 28th, 2017

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Summary July 28th, 2017

Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.

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