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Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

DOI:
10.3791/56034-v

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10:08 min

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October 24, 2017

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, , ,
1Max Planck Institute of Colloids and Interfaces

Chapters

  • 00:05Title
  • 00:44Giant Unilamellar Vescicle (GUV) Preparation
  • 03:02Phase State Assessment with Fluorescence Microscopy
  • 04:33GUV Observation and Imaging with a Microfluidic Device
  • 07:35GUV Observations at Varying Temperatures
  • 08:29Results: GUV Phase Behavior
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

Experiments on phase separated giant unilamellar vesicles (GUVs) frequently neglect physiological solution conditions. This work presents approaches to study the effect of high-salinity buffer on liquid-liquid phase separation in charged multicomponent GUVs as a function of trans-membrane solution asymmetry and temperature.

Tags

Phase BehaviorCharged VesiclesFluorescence MicroscopyAsymmetric Solution ConditionsMembrane PropertiesLipid BilayerGUVsSwelling SolutionMicrofluidic DeviceSolution Asymmetry

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