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Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta
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Developmental Biology
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JoVE Journal Developmental Biology
Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

DOI:
10.3791/56039-v

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10:57 min

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September 12, 2017

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, , , ,
1Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine,Yale University School of Medicine, 2Department of Neurology,Yale University School of Medicine, 3Department of Neurology, Xinhua Hospital,Shanghai Jiaotong University School of Medicine

Chapters

  • 00:05Title
  • 01:07In Vivo Embryonic Fate Mapping and Clonal Analysis
  • 04:14Explant Embryonic Aorta Culture
  • 05:47Smooth Muscle Cell (SMC) Perinatal Aorta Isolation
  • 07:16Subcutaneous Osmotic Mini-Pump Placement
  • 08:23Results: Representative Embryonic and Neonatal SMC Analyses
  • 09:47Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols for studying the embryonic and perinatal murine aorta using in vivo clonal analysis and fate mapping, aortic explants, and isolated smooth muscle cells are detailed here. These diverse approaches facilitate the investigation of the morphogenesis of the embryonic and perinatal aorta in normal development and the pathogenesis in disease.

Tags

Keywords: AortaVascular DevelopmentSmooth MuscleSupravalvular Aortic StenosisEmbryonicPerinatalMorphogenesisPathogenesisIn VivoCell ExplantCre ERTamoxifenProgesteroneParaformaldehydeSucroseOCT CompoundCryosectioning

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