/
/
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
This content is Free Access.
Chapters
- 00:05Title
- 01:00Negative vs Positive Sorting
- 01:51AutoMACS Bacterial Display Library Biopanning Positive Sorting
- 06:27FACS Analysis of Sorting Rounds
- 11:04Results: Representative Peptide Affinity and Specificity Date, and Alignment and Consensus Determination for Sequencing Results
- 12:46Conclusion
Biopanning bacterial display libraries is a proven technique for discovery of peptide affinity reagents, a robust alternative to antibodies. The semi-automated sorting method herein has streamlined biopanning to decrease the occurrence of false positives. Here we illustrate the thought process and techniques applied in evaluating candidates and minimizing downstream analysis.
