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High-throughput Screening for Protein-based Inheritance in S. cerevisiae

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Biology

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High-throughput Screening for Protein-based Inheritance in S. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

DOI:

10.3791/56069-v

•

08:12 min

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August 08, 2017

•

James S. Byers, Daniel F. Jarosz²

¹Department of Developmental Biology,Stanford University School of Medicine, ²Department of Chemical and Systems Biology,Stanford University School of Medicine

Chapters

00:05Title
00:33Initial Overexpression
03:12Identifying Cultures Affected by the Stressor
05:13Results: Identification of ORFs that Modulate Protein-based Inheritance
06:45Conclusion

Summary

Automatic Translation

This protocol describes a high-throughput methodology to functionally screen for protein-based inheritance in S. cerevisiae.

Tags

Keywords: High-throughput Screening Protein-based Inheritance S. Cerevisiae Prion Protein Over-expression Phenotypic Memory 96-well Plate 384-well Plate Liquid Handling Robot Optical Density Manganese Chloride SD-URA SCal-URA ST-URA

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