Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
Nanjareddy, K., Arthikala, M., Aguirre, A., Gómez, B., Lara, M. Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean. J. Vis. Exp. (130), e56140, doi:10.3791/56140 (2017).