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Immunology and Infection
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Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

DOI:
10.3791/56169-v

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06:20 min

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April 09, 2018

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, ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

Chapters

  • 00:04Title
  • 00:36Bioluminescent EHEC Bacteria Preparation for Oral Inoculation
  • 02:23Mouse Oral Gavage of EHEC
  • 03:27Data Acquisition
  • 04:46Results: Detection of Enterohemorrhagic E. coli using In Vivo Bioluminescence
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.

Tags

Enterohemorrhagic Escherichia ColiEHECColonizationMurine HostBioluminescenceIn Vivo ImagingLuciferaseKanamycinLB AgarOptical DensityStreptomycinGavageEsophagus

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