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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

DOI:
10.3791/56171-v

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12:59 min

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August 20, 2017

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, ,
1Unitat de Biologia Cellular, Facultat de Biociències,Universitat Autònoma de Barcelona

Chapters

  • 00:00Title
  • 01:08Feeder Cells Inctivation and Storage
  • 03:21Feeder Cells Culture
  • 05:14Embryo Collection and Culture
  • 06:32ESC Derivation
  • 09:06ESC Culture and Maintenance
  • 11:22Results: Validation of the Stemness and the In Vitro Differentiation Potential of the ESC Lines Established
  • 12:07Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a protocol to efficiently derive and culture pluripotent stem cell lines from mouse embryos at the blastocyst stage.

Tags

Keywords: Stem CellsEmbryonic Stem CellsMouse EmbryosPreimplantation EmbryosCell DerivationFeeder CellsFibroblastsMitomycin CInactivationCell CultureCell CountingCryopreservation

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