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Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4

DOI:
10.3791/56185-v

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09:29 min

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August 21, 2017

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, , , , , , ,
1Department of Neurology,University of California, 2Program in Immunology,University of California, 3Department of Neurology and Neurological Sciences,Stanford University, 4Department of Pathology,Stanford University

Chapters

  • 00:05Title
  • 01:04Aquaporin 4 (AQP4) Peptide Immunization
  • 03:01T Cell Cutlure and Pro-imflammatory T Cell Polarization
  • 04:35Pathogen AQP4-specific T Cell Adoptive Transfer
  • 05:29In Vivo Optical Tomography (OCT) Retinal Imaging
  • 07:11Results: Representative AQP4 Peptide-specific Th17 Cell Clinical Disease-induction Analyses
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to induce paralysis and opticospinal inflammation by transfer of aquaporin-4 (AQP4)-specific T cells from AQP4-/- mice into WT mice. In addition, we demonstrate how to use serial optical coherence tomography to monitor visual system dysfunction.

Tags

Keywords: Neuromyelitis OpticaAquaporin-4T CellsAdoptive TransferCentral Nervous System Autoimmune DiseaseOptical Coherence TomographyRetinal InjuryComplete Freund’s AdjuvantEmulsionSubcutaneous Injection

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