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Precise, High-throughput Analysis of Bacterial Growth

Article DOI: 10.3791/56197-v • 09:00 min • September 19th, 2017
September 19th, 2017 •
1, 1,2
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Institute of Biology and Information Science, East China Normal University

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Quantitative evaluation of bacterial growth is essential to understanding microbial physiology as a systems-level phenomenon. A protocol for experimental manipulation and an analytical approach are introduced, allowing for precise, high-throughput analysis of bacterial growth, which is a key subject of interest in systems biology.

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Precise High-throughput Analysis Bacterial Growth Experiment Media Strains Systems Biology Microbiology Cell Growth Determination Factors Global Parameters Maximal Growth Rate Maximal Population Density Growth Curves E. Coli Growth Micro-organisms Growth Assay Screening Environmental Bacteria Glass Tubes Silicone Rubber Stoppers Pipettes Target Strains Bunsen Burner Flame Exposure Cap Placement M63 Solution Colony Inoculation
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