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Laser Microirradiation at studere In Vivo cellulære svar til Simple og komplekse DNA-skader
JoVE Journal
Genetics
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JoVE Journal Genetics
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
DOI:

10:44 min

January 31, 2018

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Chapters

  • 00:05Title
  • 01:25Transient Transfection
  • 04:09Titration of Laser Input Power
  • 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
  • 06:54Cell Cycle and G1 Phase Synchronization
  • 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
  • 09:27Conclusion

Summary

Automatic Translation

Målet med denne protokol er at beskrive, hvordan du bruger laser microirradiation til at fremkalde forskellige typer af DNA-skader, herunder relativt simple strand pauser og komplekse skader, at studere DNA skader signalering og reparation faktor forsamling på skade steder in vivo .

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