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Journal / Genetics / Laser Microirradiation para estudar na Vivo…
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Laser Microirradiation para estudar na Vivo respostas celulares ao dano de DNA simples e complexos
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Laser Microirradiation para estudar na Vivo respostas celulares ao dano de DNA simples e complexos

Article DOI: 10.3791/56213-v • 10:44 min • January 31st, 2018
January 31st, 2018 •
*1, *2, 2, 2, 2, 2,3,4, 1
1Department of Biological Chemistry, School of Medicine, University of California, Irvine, 2Beckman Laser Institute and Medical Clinic, University of California, Irvine, 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, 4Department of Biomedical Engineering and Surgery, University of California, Irvine
* These authors contributed equally

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O objetivo do presente protocolo é descrever como usar o microirradiation do laser para induzir diferentes tipos de danos no DNA, incluindo relativamente simples quebras e danos complexos, para estudar o DNA danos reparação e sinalização fator montagem em locais de dano in vivo .

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Genética questão 131 microirradiation Laser laser em medicina danos no DNA resposta de dano do ADN reparação do DNA polimerase poli (ADP-ribose) (PARP) complexo danos quebras dano base da costa
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