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Grundtilstand udtynding super-resolution Imaging i pattedyrceller
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Grundtilstand udtynding super-resolution Imaging i pattedyrceller

Article DOI: 10.3791/56239-v • 07:55 min • November 5th, 2017
November 5th, 2017 •
1, 1, 1, 1
1Department of Physiology and Membrane Biology, University of California School of Medicine, Davis

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Denne artikel omhandler en protokol til påvisning af en eller flere plasma og/eller intracellulære Membranproteiner ved hjælp af grundtilstand udtynding (GSD) super-resolution mikroskopi i pattedyrsceller. Her diskuterer vi fordele og overvejelser til brug af sådanne tilgange til visualisering og kvantificering af cellulære proteiner.

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Strukturel biologi sag 129 CaV1.2 Konfokal mikroskopi endoplasmatiske reticulum (ER) super-opløsning total interne reflection fluorescens (JOHANNAS) mikroskopi spænding gated calcium kanaler grundtilstand udtynding mikroskopi efterfulgt af enkelte molekyle tilbage (GSDIM)
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