Journal
/
Immunology and Infection
/
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

DOI:
10.3791/56255-v

•

07:05 min

•

November 28, 2017

•

, , ,
1Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center, 2Department of Immunology,University of Texas MD Anderson Cancer Center, 3Department of Radiation Oncology,The Second Hospital of Jilin University

Chapters

  • 00:05Title
  • 01:00Administration of the Immunostimulatory Cocktail Covax
  • 01:57Isolation of Target Splenocytes for Labeling with CFSE and Peptide Pulsing of Target Splenocytes
  • 02:55Preparation of CFSE and Labeling of Target Splenocytes
  • 04:52Results: Detection of Antigen-specific T-cell Cytolytic Function
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to allow for detection of in vivo antigen-specific killing of a target cell in a murine model.

Tags

Keywords: In Vivo AssayAntigen-specific T-cell Cytolytic FunctionVaccination ModelAntigen-specific KillingCytolytic PotentialPeptide-pulsingCSFE Labeling

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56255?article_type=v

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
check_url/56255?article_type=v

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
check_url/56255?article_type=v

Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
check_url/56255?article_type=v

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
check_url/56255?article_type=v

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices
check_url/56255?article_type=v

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
check_url/56255?article_type=v

Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay
check_url/56255?article_type=v

Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
check_url/56255?article_type=v

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
check_url/56255?article_type=v

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Read Article