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Journal / Genetics / Mikroinjektion af CRISPR/Cas9 Protein i kanal…
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Genetics
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JoVE Journal Genetics
Mikroinjektion af CRISPR/Cas9 Protein i kanal havkat, Ictalurus punctatus, embryoner til Gene Editing
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Mikroinjektion af CRISPR/Cas9 Protein i kanal havkat, Ictalurus punctatus, embryoner til Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

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En enkel og effektiv mikroinjektion protokol til gen redigering i kanal havkat embryoner ved hjælp af CRISPR/Cas9 system er præsenteret. I denne protokol, guide RNA'er og Cas9 protein var microinjected i blommesækken af én-celle embryoner. Denne protokol er blevet valideret af udskære to kanal havkat immun-relaterede gener.

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Genetik sag 131 CRISPR/Cas9 gen redigering gen knockout mikroinjektion kanal havkat embryoner mutation afkortet protein indels
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