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Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

DOI:
10.3791/56282-v

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10:21 min

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October 18, 2017

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, , , ,
1Department of Neuroscience,University of Florida, 2McKnight Brain Institute,University of Florida, 3Department of Biomedical Engineering,University of Florida, 4Center for Functionally Integrative Neuroscience,Aarhus University, 5Department of Radiology,University of Florida, 6National High Magnetic Field Laboratory,Florida State University

Chapters

  • 00:05Title
  • 00:53Perfusion System Setup
  • 03:31Brain Slice Isolation
  • 04:55Sample Positioning and Purfusion System Assembly
  • 06:39Magnetic Resonance (MR) Imaging Collection
  • 07:48Results: Evaluation of aCSF Perfusion and Sample Placement for MR Microscopy
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy data.

Tags

Keywords: Magnetic Resonance MicroscopyMetabolic SupportArtificial Cerebrospinal FluidCarbogenOxygenatorPeristaltic PumpFlow RateBubble Trap

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