Journal
/
Immunology and Infection
/
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

DOI:
10.3791/56293-v

•

09:41 min

•

October 17, 2017

•

, , , ,
1Centre for Biomedical Research,Burnet Institute, 2School of Health and Biomedical Sciences,RMIT University, 3Department of Infectious Diseases,Monash University, 4University of California, Los Angeles

Chapters

  • 00:05Title
  • 00:50Culturing HUVEC Monolayer on Collagen Gels
  • 03:38Activation of HUVEC Monolayer and Isolation/Activation of Monocytes for Transmigration
  • 05:44Quantification of Foam Cells and Macrophages by Microscopy or Flow Cytometry
  • 07:42Results: Characterizing the Transmigrating Mice
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

Tags

Keywords: Monocyte TransmigrationFoam Cell FormationChronic Inflammatory ConditionsCardiovascular DiseaseAtherosclerosisEndothelial CellsCell CultureCollagen GelHUVECMicroscopyFlow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56293?article_type=v

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
check_url/56293?article_type=v

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
check_url/56293?article_type=v

Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
check_url/56293?article_type=v

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
check_url/56293?article_type=v

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
check_url/56293?article_type=v

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
check_url/56293?article_type=v

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
check_url/56293?article_type=v

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
check_url/56293?article_type=v

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages
check_url/56293?article_type=v

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Read Article