Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Thomas A. Angelovich; Anna C. Hearps; Anna Maisa; Theodoros Kelesidis; Anthony Jaworowski

doi:10.3791/56293

Journal

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Immunology and Infection

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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

13,308 Views

•

09:41 min

•

October 17, 2017

DOI:

10.3791/56293-v

DOI:

10.3791/56293-v

•

09:41 min

October 17, 2017

•

13290 Views

Thomas A. Angelovich^1,2, Anna C. Hearps^1,3, Anna Maisa¹, Theodoros Kelesidis⁴, Anthony Jaworowski^1,3

¹Centre for Biomedical Research,Burnet Institute ²School of Health and Biomedical Sciences,RMIT University ³Department of Infectious Diseases,Monash University ⁴University of California, Los Angeles

Summary

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.