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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

DOI:
10.3791/56294-v

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08:53 min

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October 02, 2017

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, , ,
1Diamond Light Source,Harwell Science & Innovation Campus, 2Research Complex at Harwell,Harwell Science & Innovation Campus

Chapters

  • 00:05Title
  • 00:42Buffer and Substrate-binding Protein (SBP) Preparation
  • 02:50SBP Crystallization and Data Collection
  • 06:19Results: Biochemical and Structural Characterization of SP0092
  • 08:07Conclusion

Summary

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Automatic Translation

A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.

Tags

Keywords: Carbohydrate TransportSubstrate-binding ProteinStreptococcus PneumoniaeBiochemical CharacterizationStructural CharacterizationBuffer StabilityOligomerizationCrystallizationSize-exclusion ChromatographyMulti-angle Light ScatteringPre-crystallization TestingProtein ConcentrationCrystallization Screening

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