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Biochemistry

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JoVE Journal Biochemistry
Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Article DOI: 10.3791/56294-v • 08:53 min • October 2nd, 2017
October 2nd, 2017 •
1,2, 1,2, 1, 1,2
1Diamond Light Source, Harwell Science & Innovation Campus, 2Research Complex at Harwell, Harwell Science & Innovation Campus

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Summary
Automatic Translation

A streamlined protocol for performing an extensive biochemical and structural characterization of a carbohydrate substrate binding protein from Streptococcus pneumoniae is presented.

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Biochemical Structural Characterization Carbohydrate Transport Substrate-binding-protein SP0092 Streptococcus Pneumoniae Instructional Studies Buffer Stability Profiles Oligomerization States Atomic Structures Crystallization Structure Solution PH Range Sodium Chloride Concentrations Microliters 96-well Plate Fluorescent Stain Pipetting Adhesive Film Centrifuge Real-time PCR System Fluorescence Readings
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