Journal
/
Developmental Biology
/
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

DOI:
10.3791/56300-v

•

08:28 min

•

April 26, 2018

•

, , ,
1Department of Biological Sciences,Delaware State University

Chapters

  • 00:05Title
  • 01:03Preparation of the Multi-electrode Array for Embryonic Neuron Culture
  • 02:11Chick Embryonic Neuron Culture
  • 05:00Recording Neuronal Network Activity
  • 07:07Results: The Effect of BPA on Synchrony Development in Chick Embryo Neuronal Cultures
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Exposure to environmental toxins can acutely impact developing embryos. Endocrine disrupting chemicals such as bisphenols are known to adversely affect the nervous system. Here we describe a protocol using an in vitro vertebrate (chick embryo) neuronal network model to study the functional impact of toxin exposure on early embryos.

Tags

Keywords: Endocrine Disrupting CompoundsNeural Network FunctionMulti-electrode ArraysVertebrate NeurobiologyDevelopmental NeurotoxicologyNetwork Spiking ActivityBisphenolsExtracellular MatrixChick Neuron IsolationForebrain Dissection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56300?article_type=v

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
check_url/56300?article_type=v

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
check_url/56300?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/56300?article_type=v

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
check_url/56300?article_type=v

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
check_url/56300?article_type=v

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
check_url/56300?article_type=v

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
check_url/56300?article_type=v

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays
check_url/56300?article_type=v

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
check_url/56300?article_type=v

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Read Article