In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Evandro F. Fang; Konstantinos Palikaras; Nuo Sun; Elayne M. Fivenson; Ryan D. Spangler; Jesse S. Kerr; Stephanie A. Cordonnier; Yujun Hou; Eszter Dombi; Henok Kassahun; Nektarios Tavernarakis; Joanna Poulton; Hilde Nilsen; Vilhelm A. Bohr

doi:10.3791/56301

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Medicine

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In Vitro og In Vivo påvisning af Mitophagy i humane celler, C. Elegansog mus

<em>In Vitro</em> and <em>In Vivo</em> Detection of Mitophagy in Human Cells,<em> C. Elegans</em>, and Mice

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Medicine

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JoVE Journal Medicine

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

In Vitro og In Vivo påvisning af Mitophagy i humane celler, C. Elegansog mus

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17,436 Views

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08:40 min

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November 22, 2017

DOI:

10.3791/56301-v

DOI:

10.3791/56301-v

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08:40 min

November 22, 2017

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14 Views

Evandro F. Fang^1,6, Konstantinos Palikaras², Nuo Sun³, Elayne M. Fivenson¹, Ryan D. Spangler⁴, Jesse S. Kerr¹, Stephanie A. Cordonnier¹, Yujun Hou¹, Eszter Dombi⁵, Henok Kassahun⁶, Nektarios Tavernarakis^2,7, Joanna Poulton⁵, Hilde Nilsen⁶, Vilhelm A. Bohr^1,8

¹Laboratory of Molecular Gerontology, National Institute on Aging,National Institutes of Health, ²Institute of Molecular Biology and Biotechnology,Foundation for Research and Technology – Hellas, ³Center for Molecular Medicine, National Heart Lung and Blood Institute,National Institutes of Health, ⁴Laboratory of Neurosciences, National Institute on Aging,National Institutes of Health, ⁵Nuffield Department of Obstetrics and Gynaecology,University of Oxford, ⁶Department of Clinical Molecular Biology,University of Oslo and Akershus University Hospital, ⁷Department of Basic Sciences, Faculty of Medicine,University of Crete, ⁸Danish Center for Healthy Aging,University of Copenhagen

Transcript

Summary

Mitophagy, processen med clearing beskadiget mitokondrier, er nødvendige for mitokondrie homøostase og sundhed vedligeholdelse. Denne artikel præsenterer nogle af de nyeste mitophagy detektionsmetoder i humane celler, Caenorhabditis elegansog mus.