Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Inriktning cystein tioler för in Vitro…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Inriktning cystein tioler för in Vitro platsspecifika Glycosylation av rekombinanta proteiner
Isolation of Leukocytes from the Human Maternal-fetal Interface
Journal (Immunology and Infection)
Isolation of Leukocytes from the Human Maternal-fetal Interface
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Journal (Immunology and Infection)
Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
 
Click here for the English version

Inriktning cystein tioler för in Vitro platsspecifika Glycosylation av rekombinanta proteiner

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Biokemiska och strukturella analyser av glykosylerat proteiner kräver relativt stora mängder homogena prover. Här presenterar vi en effektiv kemisk metod för platsspecifika glycosylation av rekombinanta proteiner renas från bakterier genom att rikta reaktiva Cys tioler.

Tags

Immunologi fråga 128 glycosylation methanethiosulfonate cystein thiol modifiering stromal interaktion molekyl-1 EFSAM lösning kärnmagnetisk resonansspektroskopi masspektrometri
Read Article
Waiting X
Simple Hit Counter