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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney
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Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney

DOI:
10.3791/56324-v

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08:26 min

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January 08, 2018

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, , , , ,
1Department of Veterans Affairs,Tennessee Valley Healthcare System, 2Departments of Medicine and Pharmacology,Vanderbilt University Medical Center, 3Department of Medicine,Baylor University College of Medicine

Chapters

  • 00:05Title
  • 00:52Preparation for Surgery
  • 01:39Injection Surgery
  • 04:03Injection Efficiency and Transgene Effects Assessment
  • 06:06Results: Hydrodynamic Renal Pelvis Injection Results in Kidney-specific Gene Expression
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.

Tags

Keywords: Hydrodynamic Renal Pelvis InjectionProtein ExpressionPlasmid DNAKidneyNephrologyVisual DemonstrationSurgical ProcedureMouseAnesthesiaIncisionKidney ExposurePlasmid DNA Injection

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