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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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JoVE Journal Cancer Research
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

DOI:
10.3791/56342-v

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11:11 min

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August 24, 2017

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, , , , , , , , ,
1Geneplus-Beijing Institute

Chapters

  • 00:05Title
  • 01:04DNA Extraction from Peripheral Blood for cfDNA and Genomics DNA (gDNA)
  • 03:02Library Preparation
  • 06:47Targeted DNA Capture
  • 09:10Results: Next Generation Sequencing to Detect Rare Mutations in ctDNA
  • 10:12Conclusion

Summary

Automatic Translation

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This manuscript describes a technique for detecting mutations of low frequency in ctDNA, ER-Seq. This method is differentiated by its unique use of two-directional error correction, a special background filter, and efficient molecular acquirement.

Tags

Keywords: Circulating Tumor DNA (ctDNA)Next-generation SequencingRare MutationsLiquid BiopsyTumor MonitoringPlasma IsolationCell-free DNA ExtractionGenomic DNA IsolationDNA Fragmentation

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