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Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C
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Een alternatieve cultuur methode om Genomic Hypomethylation van muis embryonale stamcellen met MEK-remmer PD0325901 en vitamine C

Article DOI: 10.3791/56391-v • 11:53 min • June 1st, 2018
June 1st, 2018 •
1, 1, 1
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

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Wij beschreven twee chemische-gebaseerde protocollen voor het kweken van muis embryonale stamcellen in detail. Deze nieuwe methode maakt gebruik van synergetische mechanismen ter bevordering van Tet-gemedieerde oxidatie (door vitamine C) en onderdrukken van DOVO synthese van 5-methylcytosine (door PD0325901) om DNA hypomethylation en pluripotent van muis embryonale stamcellen te onderhouden.

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Biochemie kwestie 136 vitamine C PD0325901 muis embryonale stamcellen-cellen DNA-hypomethylation Prdm14 pluripotent
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