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Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
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Chapters
- 00:05Title
- 01:23Fermentation and Sampling
- 03:52Quantification of Consumed Nitrogen Sources and Proteinogenic Amino Acids
- 05:38Measurement of Isotopic Enrichment of Proteinogenic Amino Acids
- 08:53Quantification and Isotopic Enrichment of Volatile Compounds and Calculations
- 10:21Results: Investigation of Microbial Metabolism of Multiple Nutrient Sources
- 11:38Conclusion
This protocol describes an experimental procedure to quantitatively and comprehensively investigate the metabolism of multiple nutrient sources. This workflow, based on a combination of isotopic tracer experiments and an analytical procedure, allows the fate of consumed nutrients and the metabolic origin of molecules synthetized by microorganisms to be determined.
Tags
Keywords:
Isotopic Tracer ExperimentsMicrobial MetabolismMultiple Nutrient SourcesNitrogen MetabolismYeastFermentationLabeled SubstratesProtein DistributionMetabolic OriginEnvironmental ConditionsReproducibilityPhysiological StageSynthetic MediumNitrogen SourceSaccharomyces CerevisiaeCell PopulationFermentation Platform
