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Genetics

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Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
 

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Article doi: 10.3791/56398
March 2nd, 2018

Summary March 2nd, 2018

We report a small hairpin RNA (shRNA) and next generation sequencing-based protocol for identifying regulators of X-chromosome inactivation in a murine cell line with firefly luciferase and hygromycin resistance genes fused to the methyl CpG binding protein 2 (MeCP2) gene on the inactive X chromosome.

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