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Journal / Genetics / 풀링된 shRNA MeCP2 재 활성화 비활성화 X 염색체에 대 한…
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JoVE Journal Genetics
풀링된 shRNA MeCP2 재 활성화 비활성화 X 염색체에 대 한 화면
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풀링된 shRNA MeCP2 재 활성화 비활성화 X 염색체에 대 한 화면

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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우리 보고 작은 헤어핀 RNA (shRNA) 반딧불 luciferase murine 셀 라인에서 x 염색체 비활성화의 레 귤 레이 터를 식별 하기 위한 다음 세대 시퀀싱 기반 프로토콜 및 hygromycin 저항 유전자는 메 틸 CpG 바인딩 단백질 2 (융합 MeCP2) 비활동 성 X 염색체에 유전자입니다.

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유전학 문제 133 x 염색체 비활성화 epigenetics MeCP2 레트 증후군 XIST shRNA 심사
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