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A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

DOI:
10.3791/56412-v

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10:33 min

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February 23, 2018

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, , , , , , ,
1Institute of Bioprocess and Biosystems Engineering,Hamburg University of Technology, 2Institute of Biotechnology, Department Medical Biotechnology,Technische Universität Berlin, 3TissUse GmbH

Chapters

  • 00:04Introduction
  • 00:56Agarose Gel and Collagen Cell Model Preparation
  • 02:42Permeability Assay and Diffusion Simulation Setup
  • 05:54Parameter Optimization Addition
  • 08:05Results: Representative 3D Tissue Model Analyses
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

A method for determination of permeability in a membrane insert system for multi-well plates and in silico parameter optimization for the calculation of diffusion coefficients using simulation are presented.

Tags

Membrane Insert SystemPermeabilityDiffusionOrgan-on-a-chipAgarose GelCollagen Cell ModelKeratinocytesDonor SubstanceAcceptor SubstanceFluorescence Measurement

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