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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
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Chapters
- 00:05Title
- 01:00Monomeric Avidin Fusion Protein Expression, Purification, and Refolding
- 03:53Biotinylation of BCG Cell Surface
- 05:47Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface
- 07:06Results: Biotinylation with Avidin-OVA
- 09:13Conclusion
A novel technique for rapid antigen display on a bacterial surface is presented, which involves surface biotinylation followed by exposure to proteins of interest in fusion with monomeric avidin. Loading BCG with selected antigens successfully improves its immunogenicity, suggesting that surface decoration can replace traditional genetic approaches.
