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JoVE Journal
Biochemistry

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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
 

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Article doi: 10.3791/56457
December 20th, 2017

Summary December 20th, 2017

This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.

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