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JoVE Journal
Chemistry
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resoluti...
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resoluti...
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

Full Text
9,368 Views
08:23 min
March 9, 2018

DOI: 10.3791/56465-v

Xue Li*1,2, Dan D. Huang*3, Rui Du1,2, Zhi J. Zhang1, Chak K. Chan3, Zheng X. Huang1,2, Zhen Zhou1,2

1Institute of Mass Spectrometer and Atmospheric Environment,Jinan University, 2Guangdong Provincial Engineering Research Center for On-line Source Apportionment System of Air Pollution, 3School of Energy and Environment,City University of Hong Kong

A protocol for characterizing chemical composition of exhaled breath in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry is demonstrated.

The overall goal of this experiment is to characterize chemical composition of exhaled breath using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry. This method can help to answer key questions in the medical field such as early diagnosis of diseases, therapeutic check monitoring, and environmental exposure. The main advantage of the technique is the measurement can be performed in real time.

The technique is also highest output, sensitive, specific, and user friendly. The implications of this technique have been extend to the diagnosis of lung cancer. Because chance of bio marker candidates have been find in exhaled breath of lung caner patients at different stages.

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