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Biology

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Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium
 

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Article DOI: 10.3791/56475-v 07:53 min January 16th, 2018
January 16th, 2018

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Summary

Recently we mapped the three-dimensional (3D) spatial locations of transport routes for various proteins translocating inside primary cilia in live cells. Here this paper details the experimental setup, the process of biological samples and the data analyses for the 3D super-resolution fluorescence imaging approach newly applied in live primary cilia.

Tags

High-speed Super-resolution SPEED Microscopy Live Primary Cilium Protein Transport Routes Cilia Field Subdiffraction Protein Transport NIH-3T3 Cells Cell Culture Confluency Trypsinization Culture Media Glass Bottom Plate
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