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Chapters
- 00:05Title
- 00:45Culturing, Growth, and Target-specific Staining of A. tumefaciens Strains
- 02:21Imaging of A. tumefaciens Cells
- 04:54Epifluorescence and Time-lapse Microscopy
- 05:41Results: Cells Growth of Live Labeled Microbial Cells Imaged with Fluorescence Microscopy
- 06:47Conclusion
Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.
