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JoVE Journal
Biology

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Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
 

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Article DOI: 10.3791/56497-v 07:28 min November 24th, 2017
November 24th, 2017

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Summary

Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.

Tags

Keywords: Live Cell Fluorescence Microscopy Bacterial Cell Growth Essential Processes Agrobacterium Tumefaciens Fluorescent Reagents DMSO Orange DAPI Agarose Pads Time-lapse Imaging
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