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Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth

Article DOI: 10.3791/56497-v • 07:28 min • November 24th, 2017
November 24th, 2017 •
1, 1, 1
1Division of Biological Sciences, University of Missouri

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Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.

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Live Cell Fluorescence Microscopy Essential Processes Microbial Cell Growth Bacteriology Fluorescent Reagents Bacterial Cell Surfaces Agrobacterium Tumefaciens Growth Medium Spectrophotometer OD600 Measurement Cell Culture Microfuge Tubes Centrifuge DMSO Orange Stock Solution DAPI Stock Solution
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