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Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis
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Developmental Biology
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JoVE Journal Developmental Biology
Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

DOI:
10.3791/56499-v

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09:51 min

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January 30, 2018

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, , , , ,
1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM,Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS,Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF),Biosciences and Biotechnologies Institute (BIAM)

Chapters

  • 00:05Title
  • 00:38RAW 264.7 Differentiation in the Presence of Uranyl Ions [U(VI)]
  • 03:14Osteoclast Size and Morphology Analyses
  • 05:27Osteoclast Function in the Presence of U(VI)
  • 08:30Results: Representative Effects of U(VI) on Osteoclast Size and Function
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

Uranium is known to affect bone metabolism. Here, we present a protocol aimed at investigating the effect of natural uranium exposure on the viability, the differentiation, and the function of osteoclasts, the cells in charge of bone resorption.

Tags

Keywords: OsteoclastogenesisUranium ExposureRAW 264.7 CellsCell CultureCell Seeding DensityUranium Salt SolutionRANKLOsteoclast DifferentiationOsteoclast FunctionEnvironmental ToxicityBone PhysiologyBone Metabolism

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