An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Chongfeng Xu; Junhong Ai; Qingxun Zhang; Ting Li; Xiaorong Wu; Zhengde Xie; Ziyuan Duan

doi:10.3791/56515

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Un metodo semplice ed efficace per stabilire NK e linee a cellula T da pazienti con infezione attiva cronica del Virus di Epstein - Barr

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

Un metodo semplice ed efficace per stabilire NK e linee a cellula T da pazienti con infezione attiva cronica del Virus di Epstein - Barr

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9,085 Views

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09:43 min

•

March 30, 2018

DOI:

10.3791/56515-v

DOI:

10.3791/56515-v

•

09:43 min

March 30, 2018

•

6 Views

Chongfeng Xu¹, Junhong Ai², Qingxun Zhang¹, Ting Li³, Xiaorong Wu¹, Zhengde Xie², Ziyuan Duan¹

¹Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, ²MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, ³State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

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Summary

Un metodo semplice per ottenere cloni delle cellule T e NK dai pazienti CAEBV è stato sviluppato con alta efficienza, una piccola quantità di sangue periferico e una basso-dose del-2.

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NK Cells T Cells Epstein-Barr Virus CAEBV Cell Line Establishment Cell Culture PBMC IL-2 Cell Proliferation Cell Viability

Un metodo semplice ed efficace per stabilire NK e linee a cellula T da pazienti con infezione attiva cronica del Virus di Epstein - Barr

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