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A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
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Bioengineering
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JoVE Journal Bioengineering
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

DOI:
10.3791/56523-v

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09:53 min

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January 01, 2018

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, , , ,
1Department of Otolaryngology,University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering,University of Oklahoma, 3Department of Plastic Surgery,University of Kansas Medical Center

Chapters

  • 00:05Title
  • 01:05Temporal Bone Harvest and Cochlea Isolation
  • 03:07Cochlear Processing
  • 05:11Infusion of hWJCs into Decellularized Cochleae
  • 06:58Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications.

Tags

Keywords: DecellularizationDecalcificationMouse CochleaExtracellular MatrixStem Cell DifferentiationSensory EpitheliumTemporal BoneBony LabyrinthStapesOval WindowRound WindowPerilymph1% SDS SolutionPBSAntibiotic-antimycoticPenicillin-streptomycin

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