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Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
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JoVE Journal Chemistry
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

DOI:
10.3791/56527-v

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07:12 min

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October 26, 2017

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,
1Department of Chemistry and Chemical Biology,Cornell University

Chapters

  • 00:05Title
  • 00:45Synthesis of [(OH2)(NH3)4Ru(μ-O)Ru(NH3)4(OH2)]Cl5
  • 04:11Characterization and Verification of Purity of [(OH2)(NH3)4Ru(μ-O)Ru(NH3)4(OH2)]Cl5
  • 05:19Results: Purified [(OH2)(NH3)4Ru(μ-O)Ru(NH3)4(OH2)]Cl5 as a Mitochondrial Calcium Uptake Inhibitor
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the synthesis, purification, and characterization of a ruthenium-based inhibitor of mitochondrial calcium uptake is presented. A procedure to evaluate its efficacy in permeabilized mammalian cells is demonstrated.

Tags

Keywords: RutheniumMitochondrial Calcium UptakeInhibitorSynthesisPurificationCation Exchange ChromatographyPentaammine Chloro Ruthenium DichlorideTrans-diaqua Octo Amine Mu-oxo Diruthenium Pentachloride

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