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Immunology and Infection

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JoVE Journal Immunology and Infection
Mættede fedtsyrer fremkalde ceramid-associerede makrofag celledød
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Mættede fedtsyrer fremkalde ceramid-associerede makrofag celledød

Article DOI: 10.3791/56535
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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October 31st, 2017

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Vi illustrere en ligetil metode til at udlede murine primære makrofager fra knoglemarven cellerne og en enkel metode til at forberede BSA-fedtsyre konjugater. Så vi demonstrere at mættede fedtsyrer kan fremkalde makrofag celledød, og sådanne celledød er positivt associeret med cellulære ophobning af ceramid niveauer.

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